TMB Peroxide Substrates in BIOjake. One component, ready to use
TMB is the most widely used chromogenic substrate in the
application of Horseradish peroxidase (HRP) based enzyme-linked
detection system. A stable, one component or ready to use
TMB substrate greatly reduces the assay variations at the substrate
level to standardize the assay performance. To meet the diverse
substrate requirements in the widespread HRP microwell ELISA applications and
experiments requiring visual color precipitation from
both the IVD industries and the research laboratories, the scientists
in BIOjake developed ready-to-use TMB substrates in the following categories:
1) TMB Peroxide Substrate for Microwell ELISA Assay
2) TMB Peroxide Substrate for membrane based color precipitation
Ultra fast and sensitive TMB Peroxide Substrate for Microwell ELISA Assay
TMB-UltraFast, a ready-to-use TMB substrate, is highly sensitive with ultra fast
color development and wide OD reading range at 370 nm or 620 nm to 650 nm. Adding stop solution
to increase the OD reading at 450 nm is not necessary.
Kinetic Rate selection in TMB Peroxide Substrates for Microwell ELISA Assay
In this category, TMB substrates were made in environmental friendly, 100% water solution. The end user
has the option to choose substrate of different kinetics from fast (TMB-Excellence) to slow (TMB-Slow)
and very slow (TMB-VerySlow).
The TMB-Excellence has almost equal performance as TMB-UltraFast. It has fast color development and wide OD reading range at 370 nm
or 620 nm to 650 nm. Adding stop solution to increase the OD reading at 450 nm is not necessary.
In an assay which require slow or very slow color development, the TMB-Slow or TMB-VerySlow will fit
the criteria for substrate selection. Adding Stop Solution to the test wells is able to increase OD reading at 450 nm
for 2 to 3 folds in comparison to the OD read in 370 nm or 620 nm to 650 nm.
TMB Peroxide Substrate for visual color precipitation
In the third category, TMB substrates were produced for the application of membrane based blotting assay or experiments which require
TMB substrate precipitation during the HRP enzymatic reaction. This substrate is made in environmental friendly, 100% water solution.
The HRP enzymatic reaction on this TMB substrate result deep blue color precipitation which is visible in less than a minutes.
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