TMB-VerySlow (TMBVS) is a colorless, ready to use TMB substrate to generate a soluble, deep blue color product under the enzymatic reaction with
hydrogen peroxide by HRP.
Aqueous TMB Substrate
TMB-VerySlow (TMBVS) contains no organic solvent. It's 100% water based, environmental friendly TMB substrate which
expose no safety issues with the substrate user.
Difference between TMB-VerySlowSlow and TMB-Slow
TMB-VerySlow react even slower
(see the attached fig.) than TMB-Slow in
the blue color development under HRP enzymatic reaction to offer the user who run a test requiring very slow kinetics.
Sensitivity
Reading at 650nm to track the highest OD in the microwell to reach around 1.2 to 1.5, the user then add stop solution to the plate to
read at 450nm. The signal read at 450 nm after stopping the reaction with
Stop Solution ,
is 2.5 folds stronger than the one read at 650 nm, which allows user to increase the sensitivity of the assay.
Recommended stop solution is 0.15 M sulphuric acid or 0.3 N of Hydrochloric acid.
