Stop Solution (TMB_SS) is a ready to use, acidic solution used to stop TMB substrate reactions in microwell ELISA assays. Reading at 650nm to track the highest OD in the microwell to reach around 1.2 to 1.5, the user then add stop solution to the plate to read at 450nm. The signal read at 450 nm after adding Stop Solution is 2.5 folds stronger than the one read at 650 nm, which allows user to increase the sensitivity of the assay.

Recommended stop solution is 0.15 M sulphuric acid or 0.3 N of Hydrochloric acid.

Stability and Storage

TMB-UltraFast has a shelf life of minimum of 24 months when stored at room temperature.

Large Packaging Service

TMB-SS can be ordered at large custom volume. For details on this service, please Send Us Contact.